Chelsea defender Reece James has withdrawn from the England squad due to injury and been replaced by Manchester City midfielder Nico O'Reilly who received his first senior international call-up.
James suffered the injury in Chelsea's 2-1 Premier League win over Liverpool on Saturday.
The 20-year-old O'Reilly, who has been capped at junior levels, joined Thomas Tuchel's squad for a friendly against Wales on Thursday at Wembley and a World Cup qualifier against Latvia in Riga on October 14.
Meanwhile, Spain confirmed on Monday that Manchester City midfielder Rodri Hernandez will not be joining them for their upcoming World Cup qualifiers after suffering a hamstring injury.
Rodri is "out of the squad because of injury", said the Spanish national team in a statement, ahead of games against Georgia and Bulgaria on October 11 and October 14, respectively.
The 29-year-old was taken off in the 21st minute of City’s Premier League win over Brentford on Sunday, confirming after the game he had felt discomfort in his hamstring.
Rodri was a key part of Spain's Euro 2024 triumph but suffered a serious knee injury in September of that year, missing the majority of last season as he recovered.
Spain did not say if they would be calling up another player in place of the 2024 Ballon d'Or winner.